Physiologically relevant media are associated with overlapping metabolic responses in primary human hepatocytes and Huh7 cells
RNA-seq (gene expression) · human · 30 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 30 samples: 6 conditions, mostly with about 5 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Physiologically relevant media are associated with overlapping metabolic responses in primary human hepatocytes and Huh7 cellsPhysiological reports · 2026
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE334407_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1474965 and SRA study SRP706907. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 30 samples.
How we read this: 6 replicated groups read from 30 sample titles; they account for 30 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM9788165 Huh7 cells Control 1 Huh7 cells Control
- GSM9788166 Huh7 cells Control 2 Huh7 cells Control
- GSM9788167 Huh7 cells Control 3 Huh7 cells Control
- GSM9788168 Huh7 cells Control 4 Huh7 cells Control
- GSM9788169 Huh7 cells Control 5 Huh7 cells Control
- GSM9788170 Huh7 cells OPLA 1 Huh7 cells OPLA
- GSM9788171 Huh7 cells OPLA 2 Huh7 cells OPLA
- GSM9788172 Huh7 cells OPLA 3 Huh7 cells OPLA
- GSM9788173 Huh7 cells OPLA 4 Huh7 cells OPLA
- GSM9788174 Huh7 cells OPLA 5 Huh7 cells OPLA
- GSM9788175 Huh7 cells POLA 1 Huh7 cells POLA
- GSM9788176 Huh7 cells POLA 2 Huh7 cells POLA
- GSM9788177 Huh7 cells POLA 3 Huh7 cells POLA
- GSM9788178 Huh7 cells POLA 4 Huh7 cells POLA
- GSM9788179 Huh7 cells POLA 5 Huh7 cells POLA
- GSM9788180 PHH cells Control 1 PHH cells Control
- GSM9788181 PHH cells OPLA 1 PHH cells OPLA
- GSM9788182 PHH cells POLA 1 PHH cells POLA
- GSM9788183 PHH cells Control 2 PHH cells Control
- GSM9788184 PHH cells OPLA 2 PHH cells OPLA
- GSM9788185 PHH cells POLA 2 PHH cells POLA
- GSM9788186 PHH cells Control 3 PHH cells Control
- GSM9788187 PHH cells OPLA 3 PHH cells OPLA
- GSM9788188 PHH cells POLA 3 PHH cells POLA
- GSM9788189 PHH cells Control 4 PHH cells Control
- GSM9788190 PHH cells OPLA 4 PHH cells OPLA
- GSM9788191 PHH cells POLA 4 PHH cells POLA
- GSM9788192 PHH cells Control 5 PHH cells Control
- GSM9788193 PHH cells OPLA 5 PHH cells OPLA
- GSM9788194 PHH cells POLA 5 PHH cells POLA
- GSE324107 Comparable Transcriptomic Profiles of HepaRG and HuH7 Cells Cultured in Chemically Defined and Serum-Based Media Using Primary Human Hepatocytes as Reference 20 samples shares huh7, hepatocytes, primary, media
- GSE226019 Restored glyoxylate metabolism in induced-hepatocytes after CRISPR/Cas-mediated AGXT gene correction and direct cell reprogramming of Primary Hyperoxaluria Type 1 fibroblasts 39 samples shares hepatocytes, primary, media
- GSE290042 Chromatin disruptions in pathophysiologically relevant gene promoters underlie unique SARS-Cov-2 nucleocapsid-mediated gene regulation 48 samples shares physiologically, relevant, media
- GSE227083 CDK16 and CDK18 inhibition suppresses nuclear pore-mediated DNA repair and hepatocellular carcinoma [Huh7] 33 samples shares huh7, media
- GSE282451 Gene expression profiling (total RNA-Seq) of HCV susceptibility Cells, Huh7.5 cell lines and primary human hepatocyte cells 5 samples shares huh7, primary
- GSE247407 Induction ofsteatosis in primary human hepatocytes manifests into hepatic pathophysiology of metabolic dysfunction-associated liver disease 30 samples shares hepatocytes, primary
- GSE261962 A two-step de-differentiation and maturation of primary human hepatocytes in vitro with efficient metabolic and regenerative capacities 24 samples shares hepatocytes, primary
- GSE228249 Transcriptome analysis of primary human hepatocytes-derived organoids [RNA-seq] 82 samples shares hepatocytes, primary
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.