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3' end sequencing of HeLa-NXF1-dTAG cells upon NXF1 degradation to characterize alternative polyadenylation dynamics

GSE334799 Homo sapiens Expression profiling by high throughput sequencing; Other 8 samples Submitted 2026/06/14 Platform GPL29480
Summary
Nuclear export factor 1 (NXF1) is the principal mRNA export receptor in eukaryotes. To characterize the role of NXF1 in regulating alternative polyadenylation (APA) dynamics, we employed the dTAG degradation system in a HeLa-NXF1-dTAG stable cell line. Cells were treated with 500 nM dTAG-13 or DMSO (vehicle control) for 6 hours to induce acute NXF1 depletion. Total RNA was subjected to 3' end sequencing (QuantSeq 3' mRNA-Seq Library Prep Kit V2, Lexogen) for APA analysis. Each condition was performed in biological duplicate.
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Also filed as BioProject PRJNA1476512 and SRA study SRP707999. Searching any of these in the dataset finder brings you back here.

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