Aptamer-Mediated Multivalent Endogenous ADAR1 Recruitment for Highly Efficient RNA Editing

Adenosine-to-inosine (A-to-I) RNA editing mediated by endogenous ADAR enzymes represents a promising therapeutic strategy for correcting disease-causing mutations at the transcript level without permanent genomic modification. To improve endogenous ADAR1 recruitment and RNA editing efficiency, we developed MARRS (Multivalent ADAR1 Recruiting RNA-editing System), a programmable RNA editing platform that incorporates engineered multivalent RNA aptamers targeting the Zα domain of ADAR1 p150 within a circular RNA scaffold. RNA sequencing was performed to characterize transcriptome-wide gene expression changes associated with MARRS and CLUSTER systems compared with control samples. The dataset consists of three experimental groups (Control, CLUSTER, and MARRS), each represented by three biological replicates. These data provide a resource for investigating the molecular consequences of enhanced endogenous ADAR1 recruitment and evaluating the transcriptomic impact of programmable RNA editing strategies.