GEO series

HEXB promotes tumor progression of glioblastoma through the HIF1α-CA9 pathway as a therapeutic approach

GSE334976 Homo sapiens (human) RNA-seq (gene expression) 9 samples Submitted 2026/06/30 Platform GPL24676

At a glance

RNA-seq (gene expression) · human · 9 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Topic: GlioblastomaThis series is part of our curated briefing on it.
Samples: 9 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Glioblastoma (GBM) is among the most aggressive and lethal primary malignant brain tumors, notorious for its high recurrence rates. A major challenge in GBM treatment is the paucity of patient-specific therapeutic targets, underscoring the need to identify actionable vulnerabilities that drive GBM progression. Here, through integrative proteomic and transcriptomic profiling of matched GBM tissues and non-tumor tissues adjacent to the tumor from individual patients, we identified the β-subunit of β-hexosaminidase, encoded by HEXB, as a previously underappreciated mediator of GBM progression.

Read the full summary ↓

Inhibition of HEXB markedly attenuated GBM cell proliferation and tumorigenicity. Mechanistically, HEXB interacted with HIF1α, prevented its ubiquitin-mediated degradation, and promoted its nuclear translocation, thereby enhancing the transcriptional upregulation of CA9, a hypoxia-associated enzyme implicated in glioma progression. Guided by these findings, brain-targeted nanocapsule delivery of HEXB-directed siRNA exerted therapeutic efficacy, as evidenced by robust suppression of GBM cell proliferation and tumor growth, as well as prolonged survival in mice bearing GBM xenografts. This study highlights HEXB as a promising therapeutic target for GBM and supports patient-matched proteotranscriptomic profiling as a feasible strategy for discovering vulnerabilities in GBM precision medicine.

This dataset

Open GSE334976 in finder → Find more human RNA-seq datasets → Glioblastoma landscape →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE334976_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1477032 and SRA study SRP708461. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 9 more — browse all 9 samples with per-sample file links →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.