GEO series

ChIP-seq profiling of MNDA in iPSC-derived human Sertoli-like cells

GSE335393 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 3 samples Submitted 2026/06/22 Platform GPL34284

Summary

Sertoli cells play vital roles in sustaining normal spermatogenesis. To explore the genomic binding and regulatory capacity of MNDA in Sertoli cells, we used human induced pluripotent stem cell-derived Sertoli-like cells (hiSCs) as an in vitro human Sertoli cell model, whose preparation procedures were adapted from the previously reported study (Liang et al., eLife, 2019). MNDA expression was induced in hiSCs via IFNα stimulation, and ChIP-seq was conducted to capture genome-wide MNDA binding events. Bioinformatic analysis identified thousands of specific MNDA binding peaks throughout the genome. These peaks were distributed across diverse genomic regions, including intergenic regions, gene bodies and regulatory regions flanking transcription start sites. Collectively, these results indicated that MNDA binds to multiple types of genomic loci and may modulate the transcription of downstream target genes.

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Direct links to NCBI, no account and no request form: the whole study as GSE335393_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 3 samples. Raw sequencing reads are also available from ENA.

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Also filed as BioProject PRJNA1478469 and SRA study SRP709595. Searching any of these in the dataset finder brings you back here.

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