← BioTransfer GEO Dataset Finder
GEO series

The Core Subunit NDUFS2 in Mitochondrial Complex I is Critical to Oxygen Sensing in Human Ductus Arteriosus Smooth Muscle Cells

GSE335748 Homo sapiens (human) RNA-seq (gene expression) 30 samples Submitted 2026/07/27 Platform GPL21697
At a glance

RNA-seq (gene expression) · human · 30 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
30 samples: 5 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Rationale: Mitochondria in ductus arteriosus smooth muscle cells (DASMC) are oxygen sensors triggering vasoconstriction at birth; however, the mitochondrial oxygen sensing mechanisms are not fully understood. Given the conserved role of mitochondrial Complex I subunit NDUFS2 in other oxygen-sensing tissues, we examined its role in DASMC oxygen sensing, comparing it to other Complex I subunits (NDUFS1 and NDUFS7) and putative O2-sensor subunits (UQCRFS1 and COX4I2).
Read the full summary ↓
Methods: Human DASMC were grown in hypoxia (pO2=41mmHg). Oxygen responsiveness was assessed, measuring oxygen-induced changes in intracellular calcium, [Ca2+]i, cell length, and mitochondrial reactive oxygen species (mROS) production. DASMC were treated for 48-hours with control or targeting. Knockdown was confirmed using qPCR and immunoblot. 3’RNA sequencing assessed molecular changes following siRNA manipulation. Results: Oxygen increased mitochondrial fission, [Ca2+]I, and constricted DASMC. 48-hours post-treatment, siNDUFS2 selectively depressed oxygen-induced increase in [Ca2+]i (siControl +18.6±2.3%, siNDUFS2 +5.5±1.5%, p<0.0001), DASMC shortening (from 18.4±1.1% to 8.9±0.8%, p<0.0001), and mROS (+24±4.9% untreated, -6.6±5.4% siNDUFS2, p<0.0001), without altering the KCl response or depressing respiration. The mitochondrial antioxidant MitoTEMPO reduced mROS (2.9±4.5%, p=0.001) and attenuated oxygen-induced cell shortening (8.4±0.9%, p=0.0003). Transcriptomics revealed distinct changes in mitochondrial pathways 48-hours post siNDUFS2. Conclusions: NDUFS2 regulates mROS and is a mitochondrial oxygen sensor in human DASMC.
This dataset
Open GSE335748 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE335748_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1479375 and SRA study SRP710386. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
5 conditions, mostly in triplicate
DASMC11 siNDUFS ×3 DASMC4 siNDUFS ×3 DASMC5 siNDUFS ×3 DASMC6 siNDUFS ×3 DASMC7 siNDUFS ×3 DASMC11 Control siRNA ×1 DASMC11 siUQCRFS ×1 DASMC11 siCOX4I ×1 +12 more

Supports a between-group comparison across 15 samples.

How we read this: 5 replicated groups read from 30 sample titles; they account for 15 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.