Transcriptomic profiling of GOLM1 silenced A549 lung adenocarcinoma cells

GOLM1 is a Golgi-resident protein implicated in multiple cancers, but its role in lung adenocarcinoma remains incompletely understood. RNA sequencing was performed on A549 lung adenocarcinoma cells following siRNA-mediated silencing of GOLM1 using two independent siRNAs and a non-targeting control. Differential gene expression analysis was conducted to identify molecular pathways regulated by GOLM1 depletion, including alterations in cell cycle regulation, DNA replication, lysosomal pathways, and mitochondrial-associated processes.