GEO series
Transcriptomic Signatures Reveal Overlapping Shared and DistinctYet State-Specific Preoptic Neuronal Ensembles Activated by Anesthesia and Sleep
GSE337822
Mus musculus
Expression profiling by high throughput sequencing
20 samples
2026/07/07
GPL24247
Summary
General anesthesia and non-rapid-eye-movement (NREM) sleep share overlapping neuronal circuitry within the preoptic area (POA), yet they are fundamentally distinct states. We performed single nucleus RNA sequencing (snRNA-seq) on the POA from adult C57BL/6J mice after 30-minutes of breathing either 1.2% isoflurane in oxygen or an oxygen control. Cell clusters were identified by projecting to the existing cellular taxonomy established in our previously published POA snRNA-seq dataset of recovery sleep (GEO: GSE243489) and transcriptional changes in rapid primary response genes (rPRGs) was used to define isoflurane-activated or isoflurane-inhibited clusters. Finally, multiplex fluorescence in situ hybridization was performed to independently corroborate the snRNA-seq results.
Download
NCBI GEO page ↗
{# Names what the click gives you. "Open in finder" meant nothing to a
visitor who arrived from a search engine and has never seen the tool. #}
Find more
mouse RNA-seq datasets →
Similar datasets
- GSE292862 Modelling mouse embryogenesis from chemically induced totipotent stem cells 22 samples
- GSE185862 A taxonomy of transcriptomic cell types across the isocortex and hippocampal formation 95 samples
- GSE304862 Semaglutide and exercise synergy in obesity: preserving muscle mass and uncovering organ crosstalk 209 samples
- GSE293315 MRPGRX2 Antagonist Treatment Prevents Inflammation and Disease in a Mouse Model of Atopic Dermatitis Dataset 2 35 samples
- GSE255837 Dysregulation of the Normal Wound Healing Cascade in Volumetric Muscle Loss Injury 30 samples
- GSE341948 Multi-tissue transcriptomic landscape reveals synergistic mechanisms of exercise and GLP-1 agonist in ameliorating diabetic phenotypes in db/db mice 12 samples
- GSE337190 CAR-NKT cells induce low cytokine release syndrome by targeting hyperinflammatory macrophages with mitigation from GM-SCF inhibition. 24 samples
- GSE306116 Caspase-3 Control of RNA Splicing and Mitochondrial Dynamics in Microglia during Parkinson’s Disease [RNA-Seq] 12 samples
Share this dataset
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.