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Transcriptomic profiling of ATP6AP1 knockdown in activated human hepatic stellate LX-2 cells

GSE339298 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/07/25 Platform GPL24676
Summary
Hepatic stellate cell activation is a major driver of extracellular matrix deposition during liver fibrosis. This study investigated the transcriptional changes induced by ATP6AP1 depletion in human hepatic stellate LX-2 cells. LX-2 cells were transfected with ATP6AP1-targeting siRNA or non-targeting control siRNA, with three independent biological replicates per condition. Total RNA was extracted and subjected to RNA sequencing. Sequencing reads were aligned to the human reference genome GRCh38.p10, and differential gene-expression analysis was performed using DESeq2. The dataset was generated to identify the cellular pathways regulated by ATP6AP1 depletion, with particular emphasis on hepatic stellate cell activation, cellular senescence, cell-cycle arrest and Notch signaling
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Direct links to NCBI, no account and no request form: the whole study as GSE339298_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1498110 and SRA study SRP719720. Searching any of these in the dataset finder brings you back here.

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