← BioTransfer GEO Dataset Finder
GEO series

Systematic identification of age-related microbiome metabolites that impact the brain - hCMEC screen

GSE341168 Homo sapiens (human) RNA-seq (gene expression) 138 samples Submitted 2026/07/23 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 138 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
138 samples: 40 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
These RNA-seq data were generated from immortalized hCMEC/D3 cells treated with either: (1) one of 30 blood metabolites associated with aging and the microbiome, (2) vehicle control, or (3) lipopolysaccharide (LPS), a positive control with well-characterized transcriptomic effects. For more details, please see our preprint at https://doi.org/10.1101/2025.10.03.680371.
This dataset
Open GSE341168 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE341168_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1328687 and SRA study SRP620439. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
40 conditions, mostly in triplicate
Batch2_H2O ×9 Batch3_H2O ×9 Batch1_H2O ×6 Batch4_DMSO ×6 Batch1_Cholate ×3 Batch1_DMSO ×3 Batch1_HA ×3 Batch1_IAA ×3 +32 more

Supports a between-group comparison across 138 samples.

How we read this: 40 replicated groups read from 138 sample titles; they account for 138 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 98 more — browse all 138 samples with per-sample file links →

Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.