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miR-21-5p/RasGRP1 Axis Mediates Exercise Preconditioning-Induced Microvascular Remodeling After Cerebral Ischemia-Reperfusion in Rats

GSE345907 Rattus norvegicus (rat) RNA-seq (gene expression) 9 samples Submitted 2026/09/07 Platform GPL25947
At a glance

RNA-seq (gene expression) · rat · 9 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Rattus norvegicus (rat)
Samples
9 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Regular exercise before stroke has been associated with reduced neurological injury after ischemic stroke; however, its effects on microvascular remodeling in the ischemic penumbra and the underlying molecular mechanisms remain incompletely understood. In this study, a rat middle cerebral artery occlusion/reperfusion (MCAO/R) model was established.
Read the full summary ↓
Rats underwent 3 weeks of moderate-intensity aerobic exercise preconditioning, and its effects on neurological deficits, cerebral infarct volume, TUNEL-positive cell death, and microvascular remodeling after cerebral ischemia/reperfusion injury (CIRI) were evaluated. The underlying mechanisms were investigated using integrated transcriptomic analysis and targeted molecular validation. Exercise preconditioning alleviated neurological deficits, reduced cerebral infarct volume and TUNEL-positive cell death, increased CD31-positive vascular coverage and vascular length, and enhanced the expression of endothelial junction-associated proteins, including ZO-1 and VE-cadherin. Integrated miRNA-mRNA transcriptomic analysis identified the miR-21-5p/RasGRP1/Ras/ERK signaling axis as a candidate pathway underlying the neurovascular protective effects of exercise preconditioning. Further validation demonstrated that exercise preconditioning reduced miR-21-5p expression, increased RasGRP1 expression, and enhanced Ras/ERK signaling activity. Conversely, miR-21-5p overexpression or RasGRP1 knockdown attenuated the beneficial effects of exercise preconditioning on neurological deficits, tissue injury, and microvascular remodeling. These findings suggest that exercise preconditioning promotes early neurovascular protection after CIRI, at least partly through modulation of the miR-21-5p/RasGRP1/Ras/ERK signaling axis, providing new insights into exercise-mediated stroke protection and potential therapeutic targets.
This dataset
Open GSE345907 in finder → Find more rat RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE345907_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1522373 and SRA study SRP732926. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Samples in this study

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+ 9 more — browse all 9 samples with per-sample file links →

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