GEO series

Cell division cycle in a human cancer cell line (HeLa)

GSE3497 Homo sapiens Expression profiling by array 114 samples Submitted 2005/10/22 Platform GPL2980Platform GPL3000Platform GPL2937Platform GPL3003Platform GPL3001Platform GPL3002

Summary

This experiment set contains the complete set of raw data for Whitfield et al. (2002)Mol.Biol.Cell. HeLa S3 cells were synchronized by three different methods (double thymidine block, thymidine-nocodazole block or mitotic shake-off)in five independent time courses. mRNA from the first and second double thymidine block experiments were converted to cDNA by standard methods using an oligo-dT/random hexamer mix and hybridized to 24K arrays; mRNA from the third double thymidine and thymidine-nocodazole block experiments were converted to cDNA using olido-dT alone and hybridized to 43K arrays; total RNA from the mitotic shake-off experiment was first amplified using a modified eberwine protocol, aRNA converted to cDNA by reverse transcription with random hexamer and hybridized to 43K arrays. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

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Direct links to NCBI, no account and no request form: the whole study as GSE3497_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 114 samples.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗

Also filed as BioProject PRJNA93325. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 12058064) ↗

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