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m6A-dependent regulation of messenger RNA stability

GSE49339 Homo sapiens Expression profiling by high throughput sequencing; Other 29 samples Submitted 2013/11/29 Platform GPL11154
Summary
N6-methyladenosine (m6A) is the most prevalent internal modification present in the mRNA of all higher eukaryotes. Here we present that m6A is selectively recognized by human YTH domain family (YTHDF2) protein to regulate mRNA degradation. By using crosslinking and immunoprecipitation, we have identified over 4000 substrate RNA of YTHDF2 with conserved core motif of G(m6A)C. We further estabilshed the role of YTHDF2 in RNA metabolism by a combination of ribosome profiling, RNA sequencing, m6A level quantification and cell-based imaging: the C-terminal domain of YTHDF2 selectively binds to m6A of mRNA and the N-terminal domain is responsive for localizing mRNA from translatable pool to processing body where mRNA decay occurs.
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Direct links to NCBI, no account and no request form: the whole study as GSE49339_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 29 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA213675 and SRA study SRP028325. Searching any of these in the dataset finder brings you back here.

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