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Widespread N6-methyladenosine-dependent RNA Structural Switches Regulate RNA-Protein Interactions

GSE56010 Homo sapiens Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other 18 samples Submitted 2015/02/27 Platform GPL11154
Summary
We show that N6-methyladenosine (m6A), the most abundant internal modification in mRNA/lncRNA with still poorly characterized function, alters RNA structure to facilitate the access of RBM for heterogeneous nuclear ribonucleoprotein C (hnRNP C). We term this mechanism m6A-switch. Through combining PAR-CLIP with Me-RIP, we identify 39,060 m6A-switches among hnRNP C binding sites transcriptome-wide. We show that m6A-methyltransferases METTL3 or METTL14 knockdown decreases hnRNP C binding at 16,582 m6A-switches. Taken together, 2,798 m6A-switches of high confidence are identified to mediate RNA-hnRNP C interactions and affect diverse biological processes including cell cycle regulation. These findings reveal the biological importance of m6A and provide insights into the sophisticated regulation of RNA-RBP interactions through m6A-induced RNA structural remodeling.
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Also filed as BioProject PRJNA242201 and SRA study SRP040278. Searching any of these in the dataset finder brings you back here.

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