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Human hepatocyte metaplasia in injured humanized mouse livers

GSE58679 Homo sapiens; Mus musculus Expression profiling by high throughput sequencing 13 samples Submitted 2014/09/01 Platform GPL11154Platform GPL13112Platform GPL16512
Summary
The goal of this experiment was to test whether human hepatocytes could give rise to biliary-like progenitor cells in an in vivo context. Here Fah-/- Il2ry-/- Rag2-/-NOD mouse livers were humanized with human hepatocytes. Only hepatocytes engraft in the Fah-/- mouse at detectable levels in this model. Then animals were given chronic liver injury with 0.1% ddc. After injury we measured human-specific transcripts to determine whether the phenotype of the human cells had changed. Specifically, we evaluated the relative levels of human biliary duct markers such as Spp1, Sox9, Krt7, etc. and hepatocyte markers such as Alb, Ttr, Fah, etc.
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Direct links to NCBI, no account and no request form: the whole study as GSE58679_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 13 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA253143 and SRA study SRP043434. Searching any of these in the dataset finder brings you back here.

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