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A three-dimensional map of the human genome at kilobase resolution reveals prinicples of chromatin looping

GSE63525 Homo sapiens; Mus musculus Other 200 samples Submitted 2014/12/11 Platform GPL16791Platform GPL15520Platform GPL13112Platform GPL11154Platform GPL17021
Summary
We use in situ Hi-C to probe the three-dimensional architecture of genomes, constructing haploid and diploid maps of nine cell types. The densest, in human lymphoblastoid cells, contains 4.9 billion contacts, achieving 1-kilobase resolution. We find that genomes are partitioned into local domains, which are associated with distinct patterns of histone marks and segregate into six subcompartments. We identify ~10,000 loops. These loops frequently link promoters and enhancers, correlate with gene activation, and show conservation across cell types and species. Loop anchors typically occur at domain boundaries and bind CTCF. CTCF sites at loop anchors occur predominantly (>90%) in a convergent orientation, with the asymmetric motifs ‘facing’ one another. The inactive X-chromosome splits into two massive domains and contains large loops anchored at CTCF-binding repeats.
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Also filed as BioProject PRJNA268125 and SRA study SRP050102. Searching any of these in the dataset finder brings you back here.

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