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4sU metabolic labeled transcripts in 65 YRI LCLs

GSE75220 Homo sapiens Expression profiling by high throughput sequencing 372 samples Submitted 2016/03/29 Platform GPL16791
Summary
Noncoding variants play a central role in the genetics of complex traits, but we still lack a full description of the main molecular pathways through which they act. Here we used molecular data to quantify the contribution of cis-acting genetic effects at each major stage of gene regulation from chromatin to proteins, within a population sample of Yoruba lymphoblastoid cell lines (LCLs). We performed 4sU metabolic labeled transcripts in 65 YRI LCLs to identify genetic variants that affect transcription rates. As expected, we found an important contribution of genetic variation via chromatin, contributing ∼65% of eQTLs (expression Quantitative Trait Loci). The remaining eQTLs, which are not asso- ciated with chromatin-level variation, are highly enriched in transcribed regions, and hence may affect expression through co- or post-transcriptional processes.
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Direct links to NCBI, no account and no request form: the whole study as GSE75220_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 372 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA302818 and SRA study SRP066450. Searching any of these in the dataset finder brings you back here.

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