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Systematic mapping of functional enhancer-promoter connections with CRISPR interference

GSE87257 Homo sapiens Expression profiling by high throughput sequencing 24 samples Submitted 2016/11/11 Platform GPL16791
Summary
Gene expression in mammals is regulated by noncoding elements that can impact physiology and disease, yet the functions and target genes of most noncoding elements remain unknown. We present a high-throughput approach that uses CRISPR interference (CRISPRi) to discover regulatory elements and identify their target genes. We assess >1 megabase (Mb) of sequence in the vicinity of 2 essential transcription factors, MYC and GATA1, and identify 9 distal enhancers that control gene expression and cellular proliferation. Quantitative features of chromatin state and chromosome conformation distinguish the 7 enhancers that regulate MYC from other elements that do not, suggesting a strategy for predicting enhancer-promoter connectivity. This CRISPRi-based approach can be applied to dissect transcriptional networks and interpret the contributions of noncoding genetic variation to human disease.
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Also filed as BioProject PRJNA343954 and SRA study SRP090317. Searching any of these in the dataset finder brings you back here.

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