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Perturb-seq: Dissecting molecular circuits with scalable single cell RNA profiling of pooled genetic screens

GSE90063 Mus musculus; Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2016/12/16 Platform GPL18573Platform GPL19057
Summary
Methods: We create a CRISPR vector containing a polyadenylated RNA barcode and couple it with droplet scRNA-seq to get a large scale transcriptional measurements of perturbations Results: We were able to perform regulatory inference of gene function, observe nonlinear interactions, and perform downsampling analysis to show that gene signature effects can be seen with as few as 10's of cells while gene level phenotypes, depending on effects size would require 100's of cells Conclusion: Perturb-seq presents a scalable paradigm for obtaining rich genomic profiles of perturbations
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Also filed as BioProject PRJNA354362 and SRA study SRP093670. Searching any of these in the dataset finder brings you back here.

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