← BioTransfer  ·  qPCR Primer Designer
Real-time PCR · relative quantification

qPCR Analysis

Paste your Cq / CT values → normalised fold change with statistics and a QC report. Multi-reference normalisation, ΔΔCq (Livak) or efficiency-corrected (Pfaffl). Stats are computed in Cq space, never on linear fold values.

1 · Cq data

Columns: Sample, Group, Target, Cq (order-independent; QuantStudio / CFX exports and Undetermined values are handled).

2 · Results

Results will appear here.