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RNA-seq reveals abundant circRNA, lncRNA and mRNA in blood exosomes of normal persons

GSE100206 Homo sapiens Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing 32 samples Submitted 2017/06/19 Platform GPL11154
Summary
Exosomes are small membrane vesicles of endocytic origin secreted by most cells, and contain a wealthy cargo of protein and RNA species that can modulate recipient cells’ behaviors and may be used as biomarkers for diagnosis of human diseases. They have been found in blood and are valuable sources for biomarkers due to selective cargo loading and resemblance to their parental cells. The goal of this study is to identify circRNA, lncRNA and mRNA profiles in human blood by high-throughput RNA sequencing (RNA-seq). 1-4 ml plasma or serum were used to extract exosomal RNAs by exoRNeasy Serum/Plasma Maxi kit (Qiagen). The exosomal RNAs were further treated with DNAse I and subjected to ribosome minus low-input RNAseq library preparation. The libraries were sequenced by Illumina Hiseq platform.
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Direct links to NCBI, no account and no request form: the whole study as GSE100206_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 32 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA390988 and SRA study SRP109666. Searching any of these in the dataset finder brings you back here.

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