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Cohesin loss eliminates all loop domains [ChIP-Seq]

GSE104888 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 39 samples Submitted 2017/10/13 Platform GPL16791Platform GPL18573
Summary
The human genome folds to create thousands of intervals, called “contact domains,” that exhibit enhanced contact frequency within themselves. “Loop domains” form because of tethering between two loci – almost always bound by CTCF and cohesin – lying on the same chromosome. “Compartment domains” form when genomic intervals with similar histone marks co-segregate. Here, we explore the effects of degrading cohesin. All loop domains are eliminated, but neither compartment domains nor histone marks are affected. Loss of loop domains does not lead to widespread ectopic gene activation, but does affect a significant minority of active genes. In particular, cohesin loss causes superenhancers to co-localize, forming hundreds of links within and across chromosomes, and affecting the regulation of nearby genes. We then restore cohesin and monitor the re-formation of each loop. Although re-formation rates vary greatly, many megabase-sized loops recovered in under an hour, consistent with a model where loop extrusion is rapid.
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Direct links to NCBI, no account and no request form: the whole study as GSE104888_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 39 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA414094 and SRA study SRP119849. Searching any of these in the dataset finder brings you back here.

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