GEO series

Cohesin acts as a transcriptional gatekeeper by restraining pause–release to promote processive elongation [ChIP-seq]

GSE300575 Homo sapiens (human) ChIP-seq / ATAC-seq (genome binding and open chromatin) 22 samples Submitted 2026/07/07 Platform GPL18573

At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · human · 22 samples

What it is: ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism: Homo sapiens (human)
Samples: 22 samples: 22 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Cohesin organizes 3D chromatin architecture, including promoter–enhancer loops, yet its loss has surprisingly modest effects on steady-state gene expression. We address this paradox by demonstrating that cohesin acts at different stages of transcription in human cells. First, it promotes Pol II promoter recruitment by facilitating enhancer–promoter communication that maintains active promoter chromatin states.

Read the full summary ↓

Second, it delays pause release by transiently associating with the transcriptional machinery during the pause–release transition. Kinetic modelling suggests that reduced Pol II recruitment and enhanced pause release have compensatory effects, contributing to minimal changes in steady-state gene expression across genes upon cohesin loss. In contrast, cohesin depletion impairs robust transcriptional induction in response to external stimuli. Moreover, cohesin ensures sufficient pausing duration as a quality-control-like step to promote elongation complex assembly and transcription processivity. Here, we show that cohesin regulates multiple transcriptional steps, offering mechanistic insight into cohesin-related diseases, including cancers and cohesinopathies.

This dataset

Open GSE300575 in finder → Find more human ChIP / ATAC / CUT&Tag datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE300575_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1281419 and SRA study SRP594172. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

22 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 22 sample titles: 22 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9064733 ChIPseq, BRD4, ctrl
GSM9064734 ChIPseq, BRD4, aux
GSM9064735 ChIPseq, MED1, ctrl
GSM9064736 ChIPseq, MED1, aux
GSM9064737 ChIPseq, Input, RPB1, control
GSM9064738 ChIPseq, Input, RPB1, DRB
GSM9064739 ChIPseq, Input, RPB1, release
GSM9064740 ChIPseq, RPB1, control
GSM9064741 ChIPseq, RPB1, DRB
GSM9064742 ChIPseq, RPB1, release
GSM9064743 ChIPseq, Input, control
GSM9064744 ChIPseq, Input, DRB
GSM9064745 ChIPseq, Input, release
GSM9064746 ChIPseq, RAD21, control
GSM9064747 ChIPseq, RAD21, DRB
GSM9064748 ChIPseq, RAD21, release
GSM9064749 ChIPseq, NIPBL, control
GSM9064750 ChIPseq, NIPBL, DRB
GSM9064751 ChIPseq, NIPBL, release
GSM9064752 ChIPseq, MAU2, control
GSM9064753 ChIPseq, MAU2, DRB
GSM9064754 ChIPseq, MAU2, release

Datasets on a similar topic

GSE260852 Cohesin acts as a transcriptional gatekeeper by restraining pause–release to promote processive elongation [ChIP-seq] 68 samples shares restraining, gatekeeper, processive, elongation, chip-seq, cohesin, release, promote
GSE336388 Cohesin acts as a transcriptional gatekeeper by restraining pause–release to promote processive elongation 15 samples shares restraining, gatekeeper, processive, elongation, cohesin, release, promote
GSE300577 Cohesin acts as a transcriptional gatekeeper by restraining pause–release to promote processive elongation [RNA-seq] 18 samples shares restraining, gatekeeper, processive, elongation, cohesin, release, promote
GSE300574 Cohesin acts as a transcriptional gatekeeper by restraining pause–release to promote processive elongation [TT-seq] 4 samples shares restraining, gatekeeper, processive, elongation, cohesin, release, promote
GSE265881 Tripartite phosphorylation of SPT5 by CDK9 times pause release and tunes elongation rate of RNA polymerase II (ChIP-Seq) 72 samples shares elongation, chip-seq, release
GSE294527 The USP11/TCEAL1 complex promotes transcription elongation to sustain oncogenic gene expression in neuroblastoma [ChIP-seq II] 10 samples shares elongation, chip-seq, promote
GSE280208 A CDK11-dependent RNA polymerase II pause-release checkpoint precedes CDK9-mediated transition to transcriptional elongation [ChIP-Seq] 33 samples shares elongation, chip-seq, release
GSE297204 Senataxin Promotes RNA Polymerase II Elongation [Hap1_DRBrelease_TTseq] 18 samples shares elongation, release, promote

Search all human ChIP / ATAC / CUT&Tag datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.