Single cell RNA-seq analysis of Tbx4+ and Tbx4- mouse lung mesenchymal cells

Purpose: The goals of this study are to analyze Tbx4 lineage+ and Tbx4 lineage- cells sorted from Tbx4cre;RosaTm mouse lung. Methods: Tbx4 lineage+ and Tbx4 lineage- cells within epcam+ CD31- CD45- 7AAD- cells were sorted from Tbx4cre;RosaTm mouse lung, and run on the C1 chip (Fluidigm). Cell capture, lysis, reverse transcription and cDNA amplification was performed on the C1 IFC for mRNA-seq on Fluidigm C1 Single-Cell Auto Prep System following the manufacturer’s protocol. Results: C1 single cell RNA-seq platform captured and sequenced 54 Tbx4 lineage+ cells and 39 Tbx4 lineage - cells. Conclusions: Our study analyzed transcriptomes of Tbx4 lineage+ and Tbx4 lineage- lung mesenchymal cells. This study provides a framework for the application of transcriptome profiling towards characterization of differentiated genes Tbx4 lineage+ and Tbx4 lineage - cells.