GEO series

Ribosome Profiling Reveals MK-801-Induced Differential Translation in the Prefrontal Cortex of Acute MK-801-Exposed Male Mice

GSE138616 Mus musculus (mouse) RNA-seq (gene expression) 8 samples Submitted 2026/10/01 Platform GPL21273

At a glance

RNA-seq (gene expression) · mouse · 8 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 8 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Differential translation significantly contributes to gene expression regulation and FMRP is one of the upstream translation regulators in the PFC of MK-801-exposed juvenile male mice Acute exposure to the NMDA receptor antagonist MK-801 induces schizophrenia-like behavioral endophenotypes in juvenile male mice. However, the genome-wide gene expression changes that occur at the translation level in the brains of this model are unclear.

Read the full summary ↓

Here, we performed ribosome profiling analysis on the prefrontal cortices of acute MK-801-exposed juvenile male mice. Changes in translation efficiency (TE) alone affect expression of 28.7% of genes. Only 14.1% genes were affected at only the mRNA level, and the majority of transcriptional changes were buffered at the level of translation. Genes with up-regulated TE were enriched in neural development and synapse-related gene ontologies. We observed a significantly greater number of Fragile X Mental Retardation Protein (FMRP) target genes in the TE up-regulated gene set (28.4%) than in the down-regulated gene set (15.3%). Consistently, FMRP protein was degraded in the prefrontal cortex of MK-801-exposed mice. Our findings indicate that differential translation significantly contributes to gene expression regulation, and FMRP functions as one of the upstream translation regulators in the prefrontal cortex of MK-801-exposed juvenile male mice.

This dataset

Open GSE138616 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE138616_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA576586 and SRA study SRP224920. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Samples in this study

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+ 8 more — browse all 8 samples with per-sample file links →

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