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Differential physiological, behavioral, and medial prefrontal cortex transcriptomic responses to chronic restraint stress between BALB/c and C57BL/6J mice

GSE330744 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2026/07/15 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 3 × B6 Control Group No vs 3 × B6 CRS Group No vs 3 × BALB Control Group No vs 3 × BALB CRS Group NoGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Multiple factors have been implicated in the pathology of depression and stress resilience. Nevertheless, the molecular mechanisms underlying stress responses are not yet fully elucidated. Chronic restraint stress (CRS) is widely used as a rodent model of stress-induced depressive-like phenotypes. A previous study has demonstrated strain-dependent differences in hair corticosterone and testosterone levels, as well as behavioral responses to CRS, between C57BL/6J and BALB/c mice.
Read the full summary ↓
However, the mechanisms underlying these differences remain unclear. In this study, male C57BL/6J and BALB/c mice were subjected to CRS for 6 hours per day for 21 consecutive days. Following CRS, behavioral responses were evaluated, and serum corticosterone levels along with oxidative stress-related markers were quantitatively analyzed using LC-MS/MS. In addition, comprehensive gene expression profiling of the medial prefrontal cortex was performed using next-generation sequencing, followed by Gene Ontology (GO) analysis. Control mice without CRS exposure in both strains showed increased body weight. BALB/c mice exhibited significant weight loss and elevated serum corticosterone levels, whereas C57BL/6J mice showed no change in body weight after 21 days and no significant changes in serum corticosterone levels. Oxidative stress analysis revealed a decrease in biological antioxidant potential (BAP) and an increase in derivatives of reactive oxygen metabolites (d-ROMs) in BALB/c mice, whereas C57BL/6J mice showed only an increase in d-ROMs. Behavioral analyses demonstrated that BALB/c mice exhibited increased immobility in the tail suspension test and forced swim test, as well as reduced sucrose preference following CRS. In contrast, C57BL/6J mice showed increased immobility only in the forced swim test. IPA canonical pathway analysis revealed that pathways associated with immune signaling, tissue remodeling, and metabolic processes were elevated in C57BL/6J mice compared with BALB/c mice under basal conditions. Under CRS exposure, pathways related to cell cycle progression, mitotic regulation, DNA damage response, cytoskeletal dynamics, and apoptosis were differentially regulated between the two strains. These results demonstrate marked strain-dependent differences in susceptibility and resilience to CRS. Distinct molecular programs operating at baseline and in response to stress may underlie these differences.
This dataset
Open GSE330744 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE330744_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1465357 and SRA study SRP699778. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
3 × B6 Control Group No vs 3 × B6 CRS Group No vs 3 × BALB Control Group No vs 3 × BALB CRS Group No

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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