RNA expression of human naive B cells either unstimulated or activated with CD40L in presence or absence of transient Fas engagement
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 12 samples: 3 × Control vs 3 × CD40L vs 3 × CD40L_FasL vs 3 × CD40L_FasL_QVDGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Non-apoptotic FAS signaling controls mTOR activation and extrafollicular maturation in human B cellsScience immunology · 2024
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE145513_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA607453 and SRA study SRP249980. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM4319980 Control replicate 1 Control
- GSM4319981 CD40L replicate 1 CD40L
- GSM4319982 CD40L_FasL replicate 1 CD40L_FasL
- GSM4319983 CD40L_FasL_QVD replicate 1 CD40L_FasL_QVD
- GSM4319984 Control replicate 2 Control
- GSM4319985 CD40L replicate 2 CD40L
- GSM4319986 CD40L_FasL replicate 2 CD40L_FasL
- GSM4319987 CD40L_FasL_QVD replicate 2 CD40L_FasL_QVD
- GSM4319988 Control replicate 3 Control
- GSM4319989 CD40L replicate 3 CD40L
- GSM4319990 CD40L_FasL replicate 3 CD40L_FasL
- GSM4319991 CD40L_FasL_QVD replicate 3 CD40L_FasL_QVD
- GSE284674 Comparative analysis of the cell transcriptome of primary B lymphocytes infected with the Epstein-Barr virus (EBV), its EBNA2 or EBNALP KO derivatives, or activated by CD40L and IL4 at 48 hours post-infection or treatment. 25 samples shares cd40l, activated
- GSE267099 Single-nuclei multiome (ATAC + Gene Expression) sequencing of CD40L-activated human plasmacytoid dendritic cells 2 samples shares cd40l, activated
- GSE232769 Transcriptomic analysis of human naive B cells stimulated by CD40L, CpG, anti-IgM IgG, IL-4 or combinations 36 samples shares cd40l
- GSE266889 SMART-seq2 of activated human plasmacytoid dendritic cells 383 samples shares activated
- GSE298019 PU.1-Activated Genomic Regions Define Low-risk MDS Subsets Characterized by Immune Dysregulation and Disease Progression [RNA-seq] 357 samples shares activated
- GSE300480 Effect of Activated Vitamin D on Acute Kidney Injury in Critically Ill Patients 286 samples shares activated
- GSE281592 Transcriptome Analysis in Human Breast Milk and Blood in a Randomized Trial after Inactivated or Attenuated Influenza Immunization 69 samples shares activated
- GSE273373 Mycobacterium tuberculosis resisters despite HIV exhibit activated T cells and macrophages in their pulmonary alveoli [scRNA-seq] 51 samples shares activated
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.