GEO series

Effect of Activated Vitamin D on Acute Kidney Injury in Critically Ill Patients

GSE300480 Homo sapiens (human) RNA-seq (gene expression) 286 samples Submitted 2025/08/27 Platform GPL18573

At a glance

RNA-seq (gene expression) · human · 286 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Samples: 286 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Randomized trial of activated vitamin D for acute kidney injury prevention in critically ill patientsJCI insight · 2025

Summary (written by the submitters)

Active vitamin D metabolites, including 25-hydroxyvitamin D (25D) and 1,25-dihydroxyvitamin D (1,25D), have potent immunomodulatory effects and attenuate acute kidney injury (AKI) in animal models. We conducted an NIH-funded, phase 2, randomized, double-blind, multiple-dose, 3-arm clinical trial comparing oral calcifediol (25D), calcitriol (1,25D), and placebo among critically ill adult patients at high-risk of moderate-to-severe AKI.

Read the full summary ↓

The primary end point was a hierarchical composite of death, kidney replacement therapy, and kidney injury (baseline-adjusted mean change in serum creatinine), each assessed within 7 days following enrollment using a rank-based procedure. We also performed transcriptomic analyses on circulating CD14+ monocytes collected immediately prior to randomization and two days later using RNA sequencing. The global rank score for the primary end point was similar among calcifediol vs. placebo treated patients (P=0.85) and for calcitriol vs. placebo-treated patients (P=0.58). Calcitriol upregulated more individual genes and pathways in circulating monocytes than did calcifediol, including pathways involving interferon (IFN)-alpha, IFN-gamma, oxidative phosphorylation, DNA repair, and heme metabolism. In summary, treatment with calcifediol or calcitriol in critically ill adults did not attenuate AKI, but calcitriol upregulated multiple genes and pathways involving immunomodulation, DNA repair, and heme metabolism.

Published in

Randomized trial of activated vitamin D for acute kidney injury prevention in critically ill patients

Leaf DE, Shenoy T, Zinchuk K et al. · JCI insight 2025 · PMID 40924491 · doi:10.1172/jci.insight.193523

This dataset

Open GSE300480 in finder → Find more human RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE300480_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1281035 and SRA study SRP593902. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40924491) ↗

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 286 more — browse all 286 samples with per-sample file links →

Datasets on a similar topic

GSE246206 miRNA and mRNA Signatures in Human Acute Kidney Injury Tissue 112 samples shares kidney, injury, acute
GSE302890 Discovery of kidney disease targets using multimodal human podocyte injury models 48 samples shares kidney, injury
GSE315877 Metabolic surgery mitigates early kidney injury in obese youth with diabetes by suppressing mTORC1/JAK–STAT signaling 16 samples shares kidney, injury
GSE259376 Establishment of epithelial inflammatory injury model using adult kidney organoids [RNA-seq] 16 samples shares kidney, injury
GSE242426 Hypoxic injury triggers maladaptive repair in human kidney organoids 16 samples shares kidney, injury
GSE277476 Altered bone marrow myelopoiesis contributes to kidney injury [scRNA-seq] 11 samples shares kidney, injury
GSE302560 Effect of calcium oxalate microcrystals on kidney proximal tubule epithelial cell gene expression in microgravity 144 samples shares kidney
GSE318857 Whole-Transcriptome RNA-Seq of Kidney Tissue from Patients in the Alport Spectrum 93 samples shares kidney

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.