GEO series

Human induced pluripotent stem cells free of exogenous DNA are derived with episomal vectors (fig 4.a)

GSE15176 Homo sapiens Expression profiling by array 12 samples Submitted 2009/03/27 Platform GPL570

Summary

Human induced pluripotent stem (iPS) cells have previously been derived from somatic cells using viral vectors that integrate transgenes into the genome. Genomic integration, however, can allow persistent leaky expression of the transgenes and can create insertional mutations, thus limiting the utility of these cells for both research and clinical applications. Here, we describe the derivation of human iPS cells free of vector and transgene sequences using non-integrating oriP/EBNA1-based episomal vectors. The resulting iPS cells are similar to human embryonic stem (ES) cells in both proliferative and developmental potential. These results demonstrate that reprogramming of human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors, and removes one important obstacle to the clinical applications of these cells.

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Direct links to NCBI, no account and no request form: the whole study as GSE15176_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 12 samples.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗

Also filed as BioProject PRJNA123129. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 19325077) ↗

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