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Identification of immunogenic double-stranded RNAs in human cell lines

GSE155964 Homo sapiens Expression profiling by high throughput sequencing 28 samples 2025/08/31 GPL21697GPL20795
Summary
We expressed different isoforms of ADAR proteins in human cells without A-to-I RNA editing activity to test which isoform(s) was able to rescue the interferon induction phenotypes caused by activation of MDA5 in the absense of double-stranded RNA editing. Using the rescue cells, we sequenced the RNA and profiled RNA editing in order to identify the key RNA editing substrates for MDA5 sensing.
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