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Whole transcriptomes analyses of HGC-27 cell line treated with control DMSO, disulfiram, thiram or CX-6258

GSE168618 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/06/12 Platform GPL24676
Summary
Purpose: To figure out the molecular function of disulfiram, thiram and CX-6258, RNA sequencing was performed to analyze the geome-wide change of disulfiram, thiram and CX-6258 treated HGC-27 cells compared to those of control DMSO treated cells. Methods: Total mRNA was extracted from HGC-27 cells. Then RNA quality was assessed using an Agilent Bioanalyzer 2100 and the sample reads were sequenced using Illumina Hiseq 4000 platform. As a reasult, we got the transcript data using Hisat2 followed by Stringtie. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Conclusion: The results showed disulfiram, thiram and CX-6258 may target distinct combinations of various pathways including Hippo pathway. The Hippo signaling indeed can be targeted by disulfiram, thiram and CX-6258 even though these compounds also interfere with other biological processes.
Published in
Reactivating Hippo by drug compounds to suppress gastric cancer and enhance chemotherapy sensitivity
Cao Z, Hou Y, Zhao Z et al. · The Journal of biological chemistry 2024 · PMID 38657866 · doi:10.1016/j.jbc.2024.107311
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Also filed as BioProject PRJNA713189 and SRA study SRP310089. Searching any of these in the dataset finder brings you back here.

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