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ATAC Sequencing of Murine Articular Chondrocytes Treated with TGFb1 and BMP2

GSE169380 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2026/03/25 Platform GPL21493
Summary
Murine articular chondrocytes were isolated from femoral heads of 3-week-old wild-type C57BL/6J pups as described (Gosset et al., 2008) with modifications. Following digestion, chondrocytes were harvested and cultured in complete high-glucose Dulbecco’s modified Eagle’s medium (DMEM; Gibco; #31053028) supplemented with 10% fetal bovine serum (FBS; Gibco; #10437), 2 mM L-Glutamine (Gibco; #A2916801), and 1% penicillin/streptomycin. Primary chondrocytes were seeded at a density of 50 x 104 cells/well in 12-well plates and treated with vehicle, 5 ng/ml TGFβ1 (R&D Systems; #240-B-010) and 100ng/ml BMP2 (R&D Systems; #355-BM-010) for 24 hours.
Published in
NFIA regulates articular chondrocyte fatty acid metabolism and joint homeostasis
Wang C, Fang L, Shi M et al. · Science translational medicine 2025 · PMID 40737429 · doi:10.1126/scitranslmed.adm9488
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Direct links to NCBI, no account and no request form: the whole study as GSE169380_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA716348 and SRA study SRP311702. Searching any of these in the dataset finder brings you back here.

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