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ATAC-seq in wild type and EGR2 knock-out mouse alveolar macrophages

GSE181085 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2024/08/29 Platform GPL17021
Summary
The alveolar macrophages (AMs) as a component of the innate immunity of the lung play important role in the elimination of inhaled microbes and harmful agents. The transcription factor EGR2 is a known marker transcription factor of AMs but its exact epigenetic and transcriptomic effects have not been examined. In our study, we performed ATAC-seq and RNA-seq in WT and EGR2 deficient alveolar macrophages to describe the mechanism of action and to predict potential direct target genes of EGR2. Clec7a is one of the targets of this transcription factor which is essential in the zymosan-induced inflammatory response. We further analyzed this process by applying in vivo mouse model. Our findings demonstrate that EGR2 is a key transcriptional activator, responsible for the intact protective program against different pathogens, especially fungi in AMs.
Published in
EGR2 is an epigenomic regulator of phagocytosis and antifungal immunity in alveolar macrophages
Kolostyak Z, Bojcsuk D, Baksa V et al. · JCI insight 2024 · PMID 39042472 · doi:10.1172/jci.insight.164009
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Also filed as BioProject PRJNA750658 and SRA study SRP330416. Searching any of these in the dataset finder brings you back here.

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