Triptolide sensitizes cancer cells to nucleoside DNA methyltransferase inhibitors through inhibition of DCTPP1 mediated cell-intrinsic resistance [RNA-Seq]
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 12 samples: 3 × Prostate cancer - Ctrl vs 3 × Prostate cancer - TPL vs 3 × Prostate cancer - DAC vs 3 × Prostate cancer - DACTPLGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE185650_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA770279 and SRA study SRP340829. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM5621157 Prostate cancer - Ctrl rep1 Prostate cancer - Ctrl
- GSM5621158 Prostate cancer - Ctrl rep2 Prostate cancer - Ctrl
- GSM5621159 Prostate cancer - Ctrl rep3 Prostate cancer - Ctrl
- GSM5621160 Prostate cancer - TPL rep1 Prostate cancer - TPL
- GSM5621161 Prostate cancer - TPL rep2 Prostate cancer - TPL
- GSM5621162 Prostate cancer - TPL rep3 Prostate cancer - TPL
- GSM5621163 Prostate cancer - DAC rep1 Prostate cancer - DAC
- GSM5621164 Prostate cancer - DAC rep2 Prostate cancer - DAC
- GSM5621165 Prostate cancer - DAC rep3 Prostate cancer - DAC
- GSM5621166 Prostate cancer - DACTPL rep1 Prostate cancer - DACTPL
- GSM5621167 Prostate cancer - DACTPL rep2 Prostate cancer - DACTPL
- GSM5621168 Prostate cancer - DACTPL rep3 Prostate cancer - DACTPL
- GSE185651 Triptolide sensitizes cancer cells to nucleoside DNA methyltransferase inhibitors through inhibition of DCTPP1 mediated cell-intrinsic resistance 28 samples shares dctpp1, methyltransferase, cell-intrinsic, triptolide, sensitizes, nucleoside, inhibitors, inhibition
- GSE234322 The effect of METTL3 RNA methyltransferase inhibition on breast cancer cell gene expression 18 samples shares methyltransferase, inhibition
- GSE217127 The methyltransferases METTL7A and METTL7B confer resistance of cancer cells to thiol-based histone deacetylate inhibitors (RNA-Seq) 12 samples shares methyltransferase, inhibitors
- GSE268199 Proteasome inhibition induces alteration of DNA methylation profile by attenuating the synthesis of DNA methyltransferase 1 and 3B in colorectal cancer 9 samples shares methyltransferase, inhibition
- GSE282879 Natural product inhibition of SARS-CoV-2 guanine-N7 RNA cap methyltransferase shows potent cellular antiviral efficacy 8 samples shares methyltransferase, inhibition
- GSE302665 The transcriptomic data of HaCaT cells with and without DNA methyltransferase inhibitors (DNMTi) treatment 6 samples shares methyltransferase, inhibitors
- GSE217130 The methyltransferases METTL7A and METTL7B confer resistance of cancer cells to thiol-based histone deacetylate inhibitors 22 samples shares methyltransferase, inhibitors
- GSE281811 Combined inhibition of histone methyltransferases EZH2 and DOT1L is an effective therapy for neuroblastoma 16 samples shares methyltransferase, inhibition
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.