CRISPR activation and interference screens decode stimulation responses in primary human T cells [CRISPRa Perturb-seq]
Single-cell RNA-seq · human · 16 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
- Organism
- Homo sapiens (human)
- Samples
- 16 samples
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE190604_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA787633 and SRA study SRP350148. Searching any of these in the dataset finder brings you back here.
The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.
+ 16 more — browse all 16 samples with per-sample file links →
- GSE245249 CRISPR activation screens identify the SWI-SNF ATPases as suppressors of ferroptosis 65 samples shares crispr, activation, screens
- GSE220974 CRISPRai Perturb-seq [Series 1] 16 samples shares crispr, perturb-seq, crispra
- GSE301119 Integrative analysis of pooled CRISPR screens provide functional insights into AD GWAS risk genes 28 samples shares crispr, screens
- GSE308629 Pooled CRISPR screens identify genes and non-coding genomic regions that regulate red blood cell density 99 samples shares crispr, screens
- GSE224986 Single-cell CRISPRi screens of breast cancer associated enhancers 94 samples shares crispr, screens
- GSE327424 Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cells 86 samples shares crispr, primary
- GSE271444 A CRISPR-Activation CROP-seq Screen Identifies HMGN1 as a Dosage Sensitive Regulator of Heart Defects in Down Syndrome (scRNA-Seq) 49 samples shares crispr, activation
- GSE278910 Pooled CRISPR screens with joint single-nucleus chromatin accessibility and transcriptome profiling [CUT&RUN] 30 samples shares crispr, screens
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.