GEO series

The effect of high altitudes and time-of-day on human whole-blood transcriptome

GSE196728 Homo sapiens Expression profiling by high throughput sequencing 144 samples Submitted 2022/03/03 Platform GPL24676

Summary

High altitude exposes humans to hypobaric and hypoxic conditions, which induce various physiological and molecular changes. Recent evidence, mostly from animal models, points towards interaction between circadian rhythms and the hypoxic response, however their human relevance is largely unknown. To examine the interaction between elevated altitude-low oxygen and daily rhythms, we analyzed the effect of different high altitudes in conjunction with day-time on human whole blood transcriptome. We found that high altitude vastly affects the blood transcriptome and unexpectedly, does not necessarily follow a monotonic response to altitude elevation. We observed daily variance in gene expression, which was largely dependent on altitude. Moreover, using digital cytometry approach we estimated the relative changes in abundance of different cell types and found the several immune cell types were responding in a time- and altitude-dependent manner. Taken together, our data shed new light on the transcriptional response to high altitude and its interaction with daily rhythms.

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Direct links to NCBI, no account and no request form: the whole study as GSE196728_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 144 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA806975 and SRA study SRP359813. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 35977481) ↗

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