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Nuclear Talin-1 Provides a Bridge Between Cell Adhesion and Gene Expression

GSE198191 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/01/08 Platform GPL24676
Summary
Talin-1 (TLN1) is best known to activate integrin receptors and transmit mechanical stimuli to the actin cytoskeleton at focal adhesions. However, the localization of TLN1 is not restricted to focal adhesions. By utilizing both subcellular fractionations and confocal microscopy analyses, we show that TLN1 localizes to the nucleus in several human cell lines, where it is tightly associated with the chromatin. Importantly, siRNA-mediated depletion of endogenous TLN1 triggers extensive changes in the gene expression profile of human breast epithelial cells. To determine the functional impact of nuclear TLN1, we expressed a TLN1 fusion protein containing a nuclear localization signal. Our findings revealed that accumulation of nuclear TLN1 alters the expression of a subset of genes and impairs the formation of cell-cell clusters. This study introduces an additional perspective on the canonical view of TLN1 subcellular localization and function.
Published in
Nuclear talin-1 provides a bridge between cell adhesion and gene expression
Da Silva AJ, Hästbacka HSE, Puustinen MC et al. · iScience 2025 · PMID 39898029 · doi:10.1016/j.isci.2025.111745
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Also filed as BioProject PRJNA814198 and SRA study SRP363167. Searching any of these in the dataset finder brings you back here.

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