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Spontaneous induction of neural crest cells in human pluripotent stem cell-derived aggregates revealed by single-cell RNA sequencing

GSE199158 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/01/03 Platform GPL24676
Summary
Neural crest cells (NCCs) are a transient population which contributes to the majority of craniofacial tissue. The development of craniofacial tissue involves highly complex intermingled processes of cell fate specification and migration in densely packed mesenchyme. The establishment of appropriate in vitro models would be helpful for understanding these complicated processes. Thus, we developed a new method to induce NCCs in aggregates of human pluripotent stem cells (hPSCs). To gain insight into the process of aggregate development we performed single cell RNA sequencing (scRNA-seq) on day 2, 3, 4, and 5. scRNA-seq analysis revealed that hPSCs in the aggregates acquired neural plate border NPB-like identity before NCC induction. scRNA-seq also revealed that NCCs showed changes in the gene expression associated with epithelial mesenchymal transition as in vivo developmental process.
Published in
In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells
Seto Y, Ogihara R, Takizawa K et al. · Nature communications 2024 · PMID 38355589 · doi:10.1038/s41467-024-45285-0
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Also filed as BioProject PRJNA818677 and SRA study SRP365316. Searching any of these in the dataset finder brings you back here.

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