Bulk RNA-seq of mice synovium

Purpose: Joint function is impaired by disuse. We investigated mice synovium and cartilage for control and MMS (minimized mechanical stress) model mice. Method: We developed a MMS model which could represent the disused joint by combining non-weight bearing with tail suspension and knee joint immobilization with a plastic cylinder. We followed the time course of MMS model for day 3,7,14 for synovium and day 3,7,14,28 for cartilage. In addition, we used two models to stop the immobilization; in R1, only the joint immobilization was canceled at 2 weeks, but tail suspension was continued; in R2, both the joint motion and weight bearing were allowed at 2 weeks. Conclusion: MMS synovium expressed many secreted proinflammatory cytokines. MMS cartilage expressed less cartilage anabolic genes.