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MRPGRX2 Antagonist Treatment Prevents Inflammation and Disease in a Mouse Model of Atopic Dermatitis Dataset 2

GSE293315 Mus musculus (mouse) RNA-seq (gene expression) 35 samples Submitted 2026/04/01 Platform GPL21493
At a glance

RNA-seq (gene expression) · mouse · 35 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
35 samples: 13 × skin, biopsy, HDM+SEB, INCB000262, animal vs 12 × skin, biopsy, vehicle, vehicle, animal vs 10 × skin, biopsy, HDM+SEB, vehicle, animalGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
MRGPRX2 antagonist treatment prevents inflammation and disease in a mouse model of atopic dermatitisThe Journal of investigative dermatology · 2026
Summary (written by the submitters)
The Mas-Related G-Protein Coupled Receptor X2 (MRGPRX2) is a promiscuous receptor expressed on mast cells that mediates IgE-independent mast cell activation and degranulation in response to a variety of diverse agonists and has been implicated in numerous inflammatory conditions, including atopic dermatitis. We investigated if the small molecule MRGPRX2 antagonist, INCB000262, improves disease phenotype in the house dust mite + Staphylococcus aureus enterotoxin type B (HDM+SEB) mouse model of atopic dermatitis and further characterized mast cell-mediated aspects of disease.
Read the full summary ↓
MRGPRX2 knock-in mice were sensitized to HDM+SEB and orally dosed once daily with either vehicle or INCB000262 for 4 weeks, and efficacy and skin transcriptional changes evaluated. INCB000262 attenuated HDM+SEB-induced atopic dermatitis in vivo with improvements observed in skin thickness, transepidermal water loss, tissue weight, and overall clinical score. In addition, transcriptome changes induced by HDM+SEB exhibited a mast cell activation signature consistent with human atopic dermatitis lesional skin, which was attenuated by antagonist treatment. Overall, the MRGPRX2 antagonist, INCB000262, attenuated HDM+SEB-induced atopic dermatitis disease phenotypes and mast cell-associated transcriptional changes, supporting further evaluation of INCB000262 as an oral treatment for atopic dermatitis and other mast cell-mediated diseases.
Published in
MRGPRX2 antagonist treatment prevents inflammation and disease in a mouse model of atopic dermatitis
Wollam J, Solomon M, Villescaz C et al. · The Journal of investigative dermatology 2026 · PMID 42092534 · doi:10.1016/j.jid.2026.04.021
This dataset
Open GSE293315 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE293315_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1244004 and SRA study SRP575111. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42092534) ↗
Study design
13 × skin, biopsy, HDM+SEB, INCB000262, animal vs 12 × skin, biopsy, vehicle, vehicle, animal vs 10 × skin, biopsy, HDM+SEB, vehicle, animal

Supports a between-group comparison across 35 samples.

How we read this: 3 replicated groups read from 35 sample titles; they account for 35 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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