← BioTransfer GEO Dataset Finder
GEO series

Rhinovirus-induced gene expression patterns that distinguish between neutrophilic asthma and other asthma inflammatory phenotypes in human peripheral blood mononuclear cells

GSE202216 Homo sapiens (human) RNA-seq (gene expression) 102 samples Submitted 2025/05/04 Platform GPL23227
At a glance

RNA-seq (gene expression) · human · 102 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
102 samples: 102 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Objective: The aim of the current study was to use circulating immune cells to define gene expression patterns that best distinguish between different asthma phenotypes. We hypothesised that analysing gene expression patterns in unstimulated cells would be of limited value, and that variations in gene expression patterns would be most apparent in the setting of RV stimulation.
Read the full summary ↓
Methods: Peripheral blood mononuclear cells samples from adults with persistent asthma of different inflammatory subtypes were stimulated with RV in vitro with RNA sequencing and bioinformatics used to define gene expression patterns. Results: RV-induced changes in gene expression differed markedly between neutrophilic asthma (NA) and other asthma phenotypes. Expression of many anti-viral immune pathways and their regulators genes was down regulated in NA compared to the other phenotypes, accompanied by enhanced expression of chemokine genes, proinflammatory cytokines and Y chromosome linked genes. These changes were detected in circulating, bone marrow-derived immune cells and were most apparent in RV-stimulated rather than resting cells.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE202216_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA835075 and SRA study SRP373601. Searching any of these in the dataset finder brings you back here.

Study design
102 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 102 sample titles: 102 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 62 more — browse all 102 samples with per-sample file links →

Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.