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Identifying the binding sites for miRSome on miR-9 genomic locus with ChIP-seq

GSE202645 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2024/07/01 Platform GPL24247
Summary
Recent studies have attributed multiple etiological factors for NDDs. miR-9/29a/107 is a key regulator in NDD. Therefore, understanding the upstream regulation of miR-9/29a/107 is critical for NDDs. We have used a unique multi-animal model-CC line that gives greater genetic diversity because miR upstream regulators could involve single or multiple factors. Using mirQTL mapping of CC we identified three candidates-Panx2/Polr1c/Mgea5. We showed that they modulate neuronal differentiation through miR-9. Next, we determined by ChIP that these genes are recruited to the regulatory region of the miR-9 locus. We showed that Panx2 is cleaved by RIP and translocated to the nucleus to interact with Polr1c and Mgea5. ChIP-combined-loop and 3C assays established the interaction on the miR-9 genomic locus through chromatin loops and histone modifications. We also proved that Panx2/Polr1c/Mgea5-miR-9 crosstalk to alter neuronal differentiation. Overall, we established a miRSome complex that govern transcriptional events during adult hippocampal neurogenesis.
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Direct links to NCBI, no account and no request form: the whole study as GSE202645_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA836876 and SRA study SRP374555. Searching any of these in the dataset finder brings you back here.

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