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Single-cell analysis reveals human spermatogenesis and germ cell-sertoli cell interaction

GSE202647 Homo sapiens Expression profiling by high throughput sequencing 5 samples Submitted 2025/04/23 Platform GPL24676
Summary
Non-obstructive azoospermia (NOA) is a highly heterogeneous and complex disease. However, there is lack of the research on latent biological mechanisms. Here, we performed single-cell RNA sequencing (scRNA-seq) and Assay for Transposase-Accessible Chromatin using sequencing (scATAC-seq) on patients with obstructive azoospermia and NOA. Our study identified 12 germ cell subtypes and 8 sertoli cell subtypes. Further analysis identified several stage-specific marker genes of germ cells, such as ID4, TEX19, SCML1, and DMC1 and identified several stage-specific marker genes of sertoli cells, such as SOX2, BEND2, MLC1, PGAM2, and CFH. Furthermore, Notch1/2/3 signaling and integrin were involved in the interaction between germ cells and sertoli cells. Overall, we mapped the scRNA-seq and scATAC-seq of human spermatogenesis, and analyzed germ cell and somatic cell types and their key molecular markers. It gives us a comprehensive insight in spermatogenesis and provide a fresh perspective on diagnosis and treatment for NOA.
Published in
scRNA-seq and scATAC-seq reveal that Sertoli cell mediates spermatogenesis disorders through stage-specific communications in non-obstructive azoospermia
Wang S, Wang H, Jin B et al. · eLife 2025 · PMID 40371706 · doi:10.7554/eLife.97958
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Direct links to NCBI, no account and no request form: the whole study as GSE202647_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 5 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA836879 and SRA study SRP374566. Searching any of these in the dataset finder brings you back here.

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