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The Wnt-dependent master regulator NKX1-2 controls mouse pre-implantation development

GSE202995 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/05/05 Platform GPL13112
Summary
Embryo size, specification, and homeostasis are regulated by a complex gene regulatory and signalling network. Reverse engineering of a mouse embryonic stem cell (ESC) regulatory network and its interrogation with gene expression signatures of Wnt-activated ESC clones identified NKX1-2 as a novel master regulator of pre-implantation embryo development. Nkx1-2 inhibition resulted in reduction of nascent RNA synthesis, downregulation of genes regulating ribosome biogenesis, RNA translation and transport, and in severe alteration of nucleolus structure, with exclusion of RNA polymerase I from nucleoli. In turn, these dysfunctions lead to chromosome missegregation in 2-cell to 4-cell embryo stages, severe decrease of blastomere number, alteration of tight junctions, and lack of microlumens coarsening. Taken together, these changes impair the blastocoel expansion–collapse cycle and embryo cavitation, leading to altered lineage specification and developmental arrest.
Published in
The Wnt-dependent master regulator NKX1-2 controls mouse pre-implantation development
Nakagawa S, Carnevali D, Tan X et al. · Stem cell reports 2024 · PMID 38701778 · doi:10.1016/j.stemcr.2024.04.004
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Also filed as BioProject PRJNA837982 and SRA study SRP375148. Searching any of these in the dataset finder brings you back here.

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