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The immune landscape and mechanism of hepatitis B virus surface antigen clearance [CITEseq]

GSE207541 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/07/01 Platform GPL24247
Summary
Hepatitis B virus (HBV) surface antigen (HBsAg) clearance constitutes the hallmark of resolution of acute infection and is a therapeutic goal for functional cure of chronic hepatitis B. Here, we reveal the immunological landscape and mechanism of HBsAg clearance in mice. After acquiring nonopsonized HBsAg, B cells prime CD4+ T cell responses by CCR5- and EBI2-guided interaction with CD4+ T cells at follicular and interfollicular regions, respectively. Monocyte-derived macrophages transport complement-opsonized HBsAg to follicular dendritic cells (FDCs) to maintain germinal center reaction. Batf3+ XCR1+ conventional type 1 DCs (cDC1s) acquire and present HBsAg by MHC-I cross-dressing to drive CD8+ T cell responses in liver. We map the antigen-presenting cell (APC)-T cell crosstalk landscape and identify key costimulatory signals. The immune responses in patients with acute HBV infection are revealed by a single-cell transcriptome atlas. These findings revolutionize our understanding of anti-HBV immune responses and would lead to immunotherapeutic strategies for HBsAg clearance.
Published in
Hepatitis B virus surface antigen drives T cell immunity through non-canonical antigen presentation in mice
Li X, Sun W, Xu X et al. · Nature communications 2025 · PMID 40382385 · doi:10.1038/s41467-025-59985-8
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Direct links to NCBI, no account and no request form: the whole study as GSE207541_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA856137 and SRA study SRP385071. Searching any of these in the dataset finder brings you back here.

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