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Identification and removal of unexpected proliferative off-target cells emerging after implantation of iPSC-derived pancreatic islet cells

GSE213617 Homo sapiens Expression profiling by high throughput sequencing 7 samples Submitted 2024/04/22 Platform GPL20795
Summary
The differentiation of pancreatic endocrine cells from human pluripotent stem cells (PSC) has been thoroughly investigated for their application in cell therapy against diabetes. However, there is little information available on the long-term safety of these differentiated cells. Here we show that implantation of induced PSC-derived pancreatic islet cells (iPIC) results in the appearance of unexpected proliferative off-target cells via in vivo maturation. Since the unexpected cells have characteristics of both mesenchymal stem cells (MSC) and uterine tissue, we named these cells proliferative MSC and uterus like cells (PMUC). To detect PMUC without implantation, we developed four-week-extended culture system and found that PMUC can be reduced by compound treatment, especially a taxane Docetaxel. Indeed, when we implanted Docetaxel-treated iPIC, the appearance of PMUC disappeared. The present study provides useful insights into identifying and resolving safety issues which is particularly important in the field of cell-based medicine using PSC.
Published in
Identification and removal of unexpected proliferative off-target cells emerging after iPSC-derived pancreatic islet cell implantation
Hiyoshi H, Sakuma K, Asano S et al. · Proceedings of the National Academy of Sciences of the United States of America 2024 · PMID 38598342 · doi:10.1073/pnas.2320883121
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Also filed as BioProject PRJNA881936. Searching any of these in the dataset finder brings you back here.

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