← BioTransfer GEO Dataset Finder
GEO series

VH gene accessibility in pro-B cells

GSE214081 Mus musculus Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2025/09/30 Platform GPL13112
Summary
The generation of a diverse immunoglobulin repertoire is essential for humoral immunity and requires the participation of all V genes in V(D)J recombination. Efficient repertoire formation depends on the access of approximately 100 functional VH genes spread over 2.4 Mb of the Igh locus to the DH and JH segment clusters located in the recombination center (RC) with the intronic Em enhancer. A process termed locus contraction is thought to generate proximity of the distal VH genes to the RC. Here, we explore the function of newly discovered novel enhancers and a highly transcribed VH promoter and their impact on VH gene accessibility in chromatin. We demonstrate that these transcriptional elements determines regional Igh gene transcription but have little impact on chromatin accessibility.
Published in
Exuberant long noncoding RNA expression may sculpt Igh locus topology
Drake EB, Naiyer S, Qu X et al. · Frontiers in immunology 2025 · PMID 41368627 · doi:10.3389/fimmu.2025.1678105
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE214081_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA883730 and SRA study SRP399331. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 6 more — browse all 6 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.