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Single cell gene expression profile of patient-derived head and neck squamous carcinoma cells subjected to different treatments.

GSE214146 Homo sapiens Expression profiling by high throughput sequencing 93 samples Submitted 2022/09/28 Platform GPL18573
Summary
As other tumours, HNSCC are heterogenous, hence different cell populations within a given tumour may have different response towards treatment. Most of previous studies on HNSCC resistance towards treatment have been performed on “bulk” samples, which confound the influence of its heterogenous nature. In the recent years, single cell sequencing technologies have been developing rapidly and successfully applied to investigate numerous cancer response towards treatment. A recent scRNA-seq study on HNSCC cell lines confirmed the heterogenous response towards cetuximab. To further investigate both intrinsic and acquired resistance in HNSCC, we employed two primary cells modelling different responses towards selection with EGFR inhibitor, radiation, as well as a combination of EGFR inhibitor and radiation. These cell populations were studied using RAID technology, in which RNA and protein level were simultaneously quantified at a single cell resolution.
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Direct links to NCBI, no account and no request form: the whole study as GSE214146_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 93 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA884118 and SRA study SRP399518. Searching any of these in the dataset finder brings you back here.

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