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Histone chaperon ASF1B recruits distinct epigenetic factors in regulation of histone variant H3.3 during the fetal and adult erythropoiesis.

GSE215076 Mus musculus (mouse) RNA-seq (gene expression) 54 samples Submitted 2026/05/15 Platform GPL21103
At a glance

RNA-seq (gene expression) · mouse · 54 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
54 samples: 18 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
ASF1B promotes erythropoiesis by regulating the establishment and enrichment of H3.3 nucleosomesNucleic acids research · 2026
Summary (written by the submitters)
To gain insight into ASF1B function during erythropoiesis, RNA-seq was performed in CD71/Ter119 Fluorescence-Activated Cell Sorting (FACS) cells of WT and ASF1B KO E14.5 fetal liver(S0-S5) and bone marrow(E1-E4). Notably, depletion of ASF1B in E14.5 E4/E5 fetal liver cells significantly reduced the expression of genes associated with erythroid differentiation. In contrast, depletion of ASF1B in E4 bone marrow cells significantly reduced the expression of genes enriched in chromatin assembly.
Published in
ASF1B promotes erythropoiesis by regulating the establishment and enrichment of H3.3 nucleosomes
Liu J, Song X, Zhou L et al. · Nucleic acids research 2026 · PMID 42100853 · doi:10.1093/nar/gkag447
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE215076_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA888362 and SRA study SRP401612. Searching any of these in the dataset finder brings you back here.

Study design
18 conditions, mostly in triplicate
WTS0 population ×3 WTS1 population ×3 WTS2 population ×3 WTS3 population ×3 WTS45 population ×3 KOS0 population ×3 KOS1 population ×3 KOS2 population ×3 +10 more

Supports a between-group comparison across 54 samples.

How we read this: 18 replicated groups read from 54 sample titles; they account for 54 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 14 more — browse all 54 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.