Anti-CD25 Treg targeting antibodies sparing IL-2 signaling promote regression of established glioblastoma tumors and innate cell activation.
Single-cell RNA-seq · mouse · 16 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
- Organism
- Mus musculus (mouse)
- Samples
- 16 samples: 16 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Regulatory T cell depletion promotes myeloid cell activation and glioblastoma response to anti-PD1 and tumor-targeting antibodiesImmunity · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE218013_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA901919 and SRA study SRP408075. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 16 sample titles: 16 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM6732793 Lymphoid compartment - treated with anti-CD25 - First replicate
- GSM6732794 Lymphoid compartment - treated with anti-CD25 - Used for TCRseq - First replicate
- GSM6732795 Lymphoid compartment - Untreated- First replicate
- GSM6732796 Lymphoid compartmetn - Untreated - Used for TCRseq- First replicate
- GSM6732797 Myeloid compartment - treated with anti-CD25- First replicate
- GSM6732798 Myeloid compartment - Untreated- First replicate
- GSM6732799 Tumor/stroma compartment - treated with anti-CD25- First replicate
- GSM6732800 Tumor/stroma compartment - untreated- First replicate
- GSM6732801 Lymphoid compartment - treated with anti-CD25 - Second replicate
- GSM6732802 Lymphoid compartment - treated with anti-CD25 - Used for TCRseq - Second replicate
- GSM6732803 Lymphoid compartment - Untreated- Second replicate
- GSM6732804 Lymphoid compartmetn - Untreated - Used for TCRseq- Second replicate
- GSM6732805 Myeloid compartment - treated with anti-CD25- Second replicate
- GSM6732806 Myeloid compartment - Untreated- Second replicate
- GSM6732807 Tumor/stroma compartment - treated with anti-CD25- Second replicate
- GSM6732808 Tumor/stroma compartment - untreated- Second replicate
- GSE296201 A20’s Linear Ubiquitin Binding Motif Restrains Pathogenic Activation of TH17/22 cells and IL-22 Driven Enteritis [RNA-Seq] 18 samples shares il-2, activation
- GSE296200 A20’s Linear Ubiquitin Binding Motif Restrains Pathogenic Activation of TH17/22 cells and IL-22 Driven Enteritis [ATAC-seq] 11 samples shares il-2, activation
- GSE244034 Tumor-targeting oncolytic viruses engineered for glioblastoma immunotherapy 48 samples shares glioblastoma, targeting
- GSE259346 A Private Crosstalk Established by Tumor-Targeted Cytokine Release Rescues CAR-T Activity and Engages Host T Cells against Glioblastoma 12 samples shares glioblastoma, established
- GSE313261 Global transcriptome analysis of IL-33, IL-25, IL-13, TSLP, IL-4, and IL-9 overexpression in mouse spleen 49 samples shares il-2
- GSE269681 Acidity perturbs IL-2 responsiveness, mTORC1 and c-Myc in CD8+ T cells 35 samples shares il-2
- GSE279403 Dietary cysteine enhances intestinal stemness via CD8+ T cell-derived IL-22 28 samples shares il-2
- GSE280838 IL-21 Shapes the B Cell Response in a Context-Dependent Manner [RNA-seq 27 samples shares il-2
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.